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KMID : 0360419650010010047
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Korean Journal of Pharmacology
1965 Volume.1 No. 1 p.47 ~ p.52
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A Comparative Study of Anti-inflammatory Activities of the Steriod Compounds Utilizing the "Granuloma Puch" Technic
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Abstract
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There are several methods used for screening and evaluating anti-inflammatory agents. Among these, "Granuloma pouch" technic introduced by Hans Selye is considered as a simple and reliabl method. The procedure of "Granuloma pouch" technic is as follows : Rats were used as experimental animals. An air pocket was produced in the subcutaneous tissue of the mid-dorsal portion between the shoulders by the injection of 25ml of the air which was immediately followed by injection of 1 ml of 1% croton oil as irritant. Inflammatory exudate accumulated in the pouch during the succeeding 14 days. After sacrificing the rats on the last day of the experiment, the amount of the exudate in the pouch and the weight of the granuloma tissue was measured.
The author observed and compared the anti-inflammatory activities of the several steroid compounds when they are given by different methods.
1. In the control rats, the amount of inflammatory fluid and the weight of the granuloma tissue after 14 days were 9ml and 3gm respectively.
2. Injection of hydrocortisone 1.5mg subcutanenusly, 24 hours prior to pouch formation into the area where the pouch is to be formed, successfully prevented the inflammatory processes.
3. Injection of hydrocortisone 1.5mg in the air pocket formed 24 hours prior to croton oil injection was ineffective.
4, Injection of hydrocortisone into the pouch at a distance of 5mm paprt from the pouch formation did not prevent the development of inflammation.
5. Anti-inflammatory activities of hydrocortisone administered systematically (injected intramuscularly into the area which is not related to the area of pouch formation) for 10 days were proportional to the doses of hydrocortisone administered.
6. DOCA, testosterone, and progesterone did not show the anti-inflammatory activity.
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